Center for Animal Resources and Development Database ! Home About CARD  
Strains
Genes
Reference
Disease
Download
Distribution
Home > Strains > Strain detail

Strain Detail


 - Strains   - Genes   - Reference   - Disease,Applicable field 
 Strain information
  CARD ID
2104 
Type of strain Targeted mutant.
Transgenic. 
Strain name B6;129-Pccatm1ToruTg(SAP-Pcca)47Toru 
Internal Code PCCA KO/ PCCA TG 
Submitter Miyazaki Toru 
Submitter affiliation or code Lab of Molecular Biomedicine for Pathogenesis, Center for Disease Biology and Integrative Medicine (CDBIM), The University of Tokyo 
Stock Type  
Material Transfer Conditions It is necessary to consent to the submitter. 
 
Production method In-house breading.
 
Origin
(In-house)
Organization  
Organization code  
Developer  
Origin
(From other organizations)
Organization  
Organization code  
Developer  
Year introduced  
Introduced 
Generation
 
Remarks  
 
 Gene information
 Gene information 1
  Gene symbol
Pcca 
Gene name propionyl-Coenzyme A carboxylase, alpha polypeptide 
Allele symbol tg(SAP-Pcca) 
Allele name  
MGI MGI:97499 
Chromosome  
Gene classification Gene to express(transgenic) 
Method MicroInjection 
OMIM
 
 Gene information 2
  Gene symbol
Pcca 
Gene name propionyl-Coenzyme A carboxylase, alpha polypeptide 
Allele symbol Pccatm1Timy 
Allele name propionyl-Coenzyme A carboxylase, alpha polypeptide, targeted mutation 1, Toru Miyazaki 
MGI MGI:97499 
Chromosome 14 (14E5 , 65.99) 
Gene classification Targeted or trapped gene(knockout etc.) 
Method Electroporation 
OMIM
 
 Primer 1
  PCR Primer beta-globin 5  TGC TGG TTA TTG TGC TGT CTC ATC 
PCCA 300R  AAT GGC AAC TGT CTT GAT GCC CAT 
 
 Primer 2
  PCR Primer RV13  GTA TCC ATC ATG GCT GAT GCA A 
RV14  AAC GCT ATG TCC TGA TAG CGG T 
 

 Reference information
 reference information 1
  Author
Miyazaki T, Ohura T, Kobayashi M, Shigematsu Y, Yamaguchi S, Suzuki Y, Hata I, Aoki Y, Yang X, Minjares C, Haruta I, Uto H, Ito Y, Müller U 
Title Fatal propionic acidemia in mice lacking propionyl-CoA carboxylase and its rescue by postnatal, liver-specific supplementation via a transgene. 
Journal J Biol Chem 
Volume 276 
Page 35995-9 
Year 2001 
PMID  
 
 Disease , Applicable field information
  Disease name,
Applicable field
Metabolism