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Strain Detail

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Strain information

	CARD ID		2091
	Type of strain		Targeted mutant.
	Strain name		B6;129-Rab8a^tm1Rab8b^tm1
	Internal Code		Rab8ab DKO
	Submitter		- -
	Submitter affiliation or code		-
	Stock Type
	Material Transfer Conditions		It is necessary to consent to the submitter.
	Production method		In-house breading.
	Origin (In-house)	Organization
		Organization code
		Developer
	Origin (From other organizations)	Organization
		Organization code
		Developer
		Year introduced
		Introduced Generation
	Remarks		Rab8a+/-; Rab8b-/-

Gene information

Gene information 1

	Gene symbol		Rab8b
	Gene name		Rab8b, member RAS oncogene
	Allele symbol		Rab8b^tm1
	Allele name		Rab8b, member RAS oncogene, targeted mutation 1
	MGI		MGI:2442982
	Chromosome		9 (36.17)
	Gene classification		Targeted or trapped gene(knockout etc.)
	Method		Electroporation
	OMIM

Gene information 2

	Gene symbol		Rab8a
	Gene name		Rab8a, member RAS oncogene
	Allele symbol		Rab8a^tm1
	Allele name		Rab8a, member RAS oncogene, targeted mutation 1
	MGI		MGI:96960
	Chromosome		8 (34.84)
	Gene classification		Targeted or trapped gene(knockout etc.)
	Method		Electroporation
	OMIM

Primer 1

	PCR Primer	8a genotype E	tgg ctt atg gca tat ctg ctg g
		8a genotype A	aag cag gcc tca act cac tga gat c

Primer 2

	PCR Primer	8a genotype E	tgg ctt atg gca tat ctg ctg g
		8a genotype R	gcc aag ttt ggg gca tag tta c

Primer 3

	PCR Primer	8bWT-S	gtt gga cat ctc ttc acc tat gc
		8bNull-AS	cag agc ctg aga agg tgc aat g

Reference information

reference information 1

	Author	Sato T, Mushiake S, Kato Y, Sato K, Sato M, Takeda N, Ozono K, Miki K, Kubo Y, Tsuji A, Harada R, Harada A.
	Title	The Rab8 GTPase regulates apical protein localization in intestinal cells.
	Journal	Nature
	Volume	448
	Page	366-369
	Year	2007
	PMID

reference information 2

	Author	Takashi Sato, Tomohiko Iwano, Masataka Kunii, Shinji Matsuda, Rumiko Mizuguchi, Yongwook Jung, Haruo Hagiwara, Yoshihiro Yoshihara, Michisuke Yuzaki, Reiko Harada and Akihiro Harada
	Title	Rab8a and Rab8b are essential for multiple apical transport pathways but insufficient for ciliogenesis
	Journal	Journal of Cell Science
	Volume
	Page
	Year	in press
	PMID

Disease , Applicable field information

Disease name,
Applicable field

Digestive Disorders, Metabolism, Neurobiology, Cell biology, Anatomy, Physiology