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Strain Detail

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Strain information

	CARD ID		2226
	Type of strain		Targeted mutant.
	Strain name		B6;129-Prkd1^tm1 Prkd2^tm1
	Internal Code		B6;129-Prkd1 flox, Prkd2 flox
	Submitter		- -
	Submitter affiliation or code		-
	Stock Type
	Material Transfer Conditions		It is necessary to consent to the submitter.
	Production method		In-house breading.
	Origin (In-house)	Organization	I.M.C.R., Gunma Univ,
		Organization code
		Developer	Akihiro Harada
	Origin (From other organizations)	Organization
		Organization code
		Developer
		Year introduced
		Introduced Generation
	Remarks

Gene information

Gene information 1

	Gene symbol		Prkd1
	Gene name		Protein Kinase D1
	Allele symbol		Prkd1^tm1
	Allele name		Protein Kinase D1, targeted mutation 1
	MGI		MGI:99879
	Chromosome		12 (21.78)
	Gene classification		Targeted or trapped gene(knockout etc.)
	Method		Electroporation
	OMIM

Gene information 2

	Gene symbol		Prkd2
	Gene name		Protein kinase D2
	Allele symbol		Prkd2^tm1
	Allele name		Protein kinase D2, targeted mutation 1
	MGI		MGI:2141917
	Chromosome		7 (9.15)
	Gene classification		Targeted or trapped gene(knockout etc.)
	Method		Electroporation
	OMIM

Primer 1

	PCR Primer	Geo F	CATCATCACACCCATGCCCTCAAGT
		WT R	CCCAGTTACTCCAAAGTGATTCAAT

Primer 2

	PCR Primer	intron1 F	TCTATTTAGCCCAGCCCCCTCCCACT
		intron1 R	TGGCCCAGCATTGGGGCAGAATGAA

Reference information

reference information 1

	Author	Nur Atik, Masataka Kunii, Erda Avriyanti, Naomi Furumoto, Keiko Inami, Shin-ichiro Yoshimura, Reiko Harada, and Akihiro Harada
	Title	The Role of PKD in Cell Polarity, Biosynthetic Pathways, and Organelle/F-actin Distribution.
	Journal	Cell Structure and Function
	Volume	39
	Page	61-77
	Year	2014
	PMID

reference information 2

	Author	Avriyanti E, Atik N, Kunii M, Furumoto N, Iwano T, Yoshimura SI, Harada R, Harada A.
	Title	Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity.
	Journal	Neuroscience Research
	Volume	in press
	Page
	Year	2015
	PMID

Disease , Applicable field information

Disease name,
Applicable field

Hematology, Cell biology, Anatomy