| |
CARD ID
 |
2104 |
| Type of strain |
Targeted mutant.
Transgenic. |
| Strain name |
B6;129-Pccatm1ToruTg(SAP-Pcca)47Toru |
| Internal Code |
PCCA KO/ PCCA TG |
| Submitter |
Miyazaki Toru |
| Submitter affiliation or code |
Lab of Molecular Biomedicine for Pathogenesis, Center for Disease Biology and Integrative Medicine (CDBIM), The University of Tokyo |
| Stock Type |
|
| Material Transfer Conditions |
It is necessary to consent to the submitter.
|
| Production method |
In-house breading.
|
Origin
(In-house) |
Organization |
|
| Organization code |
|
| Developer |
|
Origin
(From other organizations) |
Organization |
|
| Organization code |
|
| Developer |
|
| Year introduced |
|
Introduced
Generation |
|
| Remarks |
|
| |
Gene symbol
|
Pcca |
| Gene name |
propionyl-Coenzyme A carboxylase, alpha polypeptide |
| Allele symbol |
tg(SAP-Pcca) |
| Allele name |
|
| MGI |
MGI:97499 |
| Chromosome |
|
| Gene classification |
Gene to express(transgenic) |
| Method |
MicroInjection |
| OMIM |
|
|
| |
Gene symbol
|
Pcca |
| Gene name |
propionyl-Coenzyme A carboxylase, alpha polypeptide |
| Allele symbol |
Pccatm1Timy |
| Allele name |
propionyl-Coenzyme A carboxylase, alpha polypeptide, targeted mutation 1, Toru Miyazaki |
| MGI |
MGI:97499 |
| Chromosome |
14 (14E5 , 65.99) |
| Gene classification |
Targeted or trapped gene(knockout etc.) |
| Method |
Electroporation |
| OMIM |
|
|
| |
PCR Primer |
beta-globin 5
|
TGC TGG TTA TTG TGC TGT CTC ATC
|
|
PCCA 300R
|
AAT GGC AAC TGT CTT GAT GCC CAT
|
|
| |
PCR Primer |
RV13
|
GTA TCC ATC ATG GCT GAT GCA A
|
|
RV14
|
AAC GCT ATG TCC TGA TAG CGG T
|
|
| reference information 1 |
| |
Author
|
Miyazaki T, Ohura T, Kobayashi M, Shigematsu Y, Yamaguchi S, Suzuki Y, Hata I, Aoki Y, Yang X, Minjares C, Haruta I, Uto H, Ito Y, Müller U |
| Title |
Fatal propionic acidemia in mice lacking propionyl-CoA carboxylase and its rescue by postnatal, liver-specific supplementation via a transgene. |
| Journal |
J Biol Chem |
| Volume |
276 |
| Page |
35995-9 |
| Year |
2001 |
|
PMID
|
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|
Disease , Applicable field information
|
| |
Disease name,
Applicable field
|
Metabolism
|
|
|
