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Strain Detail


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 Strain information
  CARD ID
257 
Type of strain Transgenic. 
Strain name C57BL/6-Tg(TCR-OT-II.2)Cbn 
Internal Code C57BL/6-TgN(TCR-OT-?)cbn, OT-II
C57BL/6-TgN(TCR-OT-?)Cbn 
Submitter Shigeo Koyasu 
Submitter affiliation or code RIKEN 
Stock Type  
Material Transfer Conditions It is necessary to consent to the submitter. 
 
Production method From other organizations.
 
Origin
(In-house)
Organization  
Organization code  
Developer  
Origin
(From other organizations)
Organization Walter Eliza Hall Institute 
Organization code  
Developer William R. Heath 
Year introduced April 2001  
Introduced 
Generation
N? 
Remarks  
 
 Gene information
 Gene information 1
  Gene symbol
? TCRAV2 TCRBV5 ? 
Gene name V alpha 2J alpha ?@V beta 5D beta ? J beta ? 
Allele symbol  
Allele name  
MGI  
Chromosome  
Gene classification Gene to express(transgenic) 
Method  
OMIM
 
 Primer 1
  PCR Primer primerA  PCR primers used to amplify the transgenic TCR α-chain are:
Vα2-5' 5'-AAA GGG AGA AAA AGC TCT CC-3'
Cαb 5'-ACA CAG CAG GTT CTG GGT TC-3'
which amplify a 160 bp fragment(from Casanova et al., 1991, J.Exp. Med. 174:1371) 
primerB  As an internal control, primers to insulin can also used. These are:
MINSIXHOF-5' 5'-CGA GCT CGA GCC TGC CTA TCT TTC AGG TC-3'
MINSBAMR-5' 5'-CGG GAT CCT AGT TGC AGT AGT TCT CCA G-3'
which amplify a 380 bp fragment of the insulin gene. 
 

 Reference information
 reference information 1
  Author
MEGAN J BARNDEN, JAN ALLISON, WILLIAM R HEATH and FRANCIS R CARBONE 
Title Defective TCR expression in transgenic mice constructed using cDNA-based α- and β-chain genes under the control of heterologous regulatory elements 
Journal Immunology and Cell Biology 
Volume 76 
Page 34-40 
Year 1998 
PMID 9553774  
 
 Disease , Applicable field information
  Disease name,
Applicable field
Immunology