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Strain Detail


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 Strain information
  CARD ID
3084 
Type of strain Targeted mutant. 
Strain name C57BL/6-Zfp541tm1 REC8tm1(3XFLAG-HA-p2A-GFP) 
Internal Code Zfp541#16/REC8-3XFLAG-HA-p2A-GFP KI 
Submitter Ishiguro Kei-ichiro 
Submitter affiliation or code Department of Chromosome Biology, Institute of Molecular Embryology and Genetics,Kumamoto University 
Stock Type  
Material Transfer Conditions It is necessary to consent to the submitter. 
 
Production method In-house breading.
 
Origin
(In-house)
Organization  
Organization code  
Developer  
Origin
(From other organizations)
Organization  
Organization code  
Developer  
Year introduced  
Introduced 
Generation
 
Remarks  
 
 Gene information
 Gene information 1
  Gene symbol
Rec8 
Gene name REC8 meiotic recombination protein 
Allele symbol  
Allele name  
MGI  
Chromosome 14 (28.19) 
Gene classification Targeted or trapped gene(knockout etc.) 
Method Electroporation 
OMIM
 
 Primer 1
  PCR Primer 68_Rec8-Left2-F  GTCGACTGAGCAAGTTCATTAAACCATATCCTG 
85_Rec8-Rightarm-R  GCGGCCGCTCTTGAAGCTGACATCTTTGGTTAC 
 
 Primer 2
  PCR Primer ZFP541-F1  agctagctgccagcgagggctcttc 
ZFP541-R2  tggttgagtgtgtcactgcagttgag 
ZFP541-R3  gaggcagcagaagggaggtaggatg 
 

 Reference information
 reference information 1
  Author
Horisawa-Takada Y, Kodera C, Takemoto K, Sakashita A, Horisawa K, Maeda R, Usuki S, Fujimura S, Tani N, Matsuura K, Shimada R, Akiyama T, Suzuki A, Niwa H, Tachibana M, Ohba T, Katabuchi H, Namekawa S, Araki K, Ishiguro K 
Title Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis 
Journal Nature Communications 
Volume In press 
Page  
Year 2021 
PMID  
 
 Disease , Applicable field information
  Disease name,
Applicable field
Reproduction, Cell biology, Molecular biology